In Vitro Studies in Enicostema Littorale Blume

Enicostema littorale is popularly known as Indian Whitehead belongs to family gentiaceae. It is a perennial herb distributed in West Indies, tropical Africa, India and Sri Lanka. It is found almost throughout India up to an altitude of about 450 meter. The whole plant of Indian whitehead used in the treatment of diabetes mellitus, rheumatism, abdominal ulcers, hernia, swelling, itching, malaria and insect poisoning. During present piece of work efforts have been made to regenerate this important medicinal plant in vitro. Explants viz. shoot tip, leaves and stem were cultured on MS medium, with different concentration of BAP, KIN, NAA, and IBA. Induction of callus was recorded on MS fortified with BAP 1.5 mg/lit along with IAA 1.5 mg/lit. Friable callus was noticed after 15 days.