High Frequency Regeneration in Important Medicinal Plant Uraria PICTA JACQ DC

Present investigation is aimed to develop the high frequency regeneration in Uraria picta. This plant is overexploited for preparation of the drug Dashmula. Explants used during investigation were axillary buds, segment of leaves and nodal segments which responded well to all growth regulators. High frequency regeneration of shoots was achieved within 21 days on modified Murashige and Skoog's medium supplemented with 0.5 mg/L of Indole-3-acetic acid along with 2.5 mg/L 6-benzylaminopurine. Isolated shoots rooted on MS along with in 0.5 mg/L indole-3-acetic acid. In vitro raised plants after hardening were grew normally in soil after treatment with inorganic salt solution.