Immunomodulatory Effect of Triphala Bio fortification in Oreochromis mossambicus

The present study was conducted to evaluate the bio fortification efficacy of methanolic extract of Triphala in Oreochromis mossambicus as reflected by haematological parameters. Haematological parameters like total erythrocyte count (TEC), total leucocyte count (TLC), differential leucocyte count (DLC), haemoglobin (Hb), hematocrit, (Ht), mean cell haemoglobin (MCH) serum total protein, albumin and globulin were analyzed in Triphala (0.8 %) fortified O. mossambicus subsequently challenged with Aeromonas hydrophila. Significant increase in TEC, TLC, Hb, Ht, MCH and serum total protein and albumin were observed in Triphala supplementation group compared to control and increase in globulin level was insignificant. Post Aeromonas hydrophila infection, TLC, DLC and survival status improved in Triphala fortified group compared to control. Hence present study confirms that Triphala (0.8 %), supplementation in fish feed acts as an immunomodulator.