Features of Reproduction Rare and Endangered Species of Lagochilus Proskorjakovii Ikramov in Culture in Vitro
Journal: International Journal of Science and Research (IJSR) (Vol.5, No. 9)Publication Date: 2016-09-05
Authors : Abdullaeva D.A; Safarov K.S;
Page : 1527-1529
Keywords : L proskorjakovii; culture in vitro; micropropagation; root formation;
Abstract
The features of micro propagation herb Lagohilus proskorjakovii Ikramov - rare endemic of Nuratau mountain. The conditions for the introduction of rare and endangered plant species L. proskorjakovii sterile in vitro culture the method of sterilization, type of primary explants, saline environments and hormonal preparations. We investigated a number of culture media for culturing to determine the most optimal to maintain the culture. It is shown that only when the sterilization of seeds L. proskorjakovii in solutions of AgNO3 0.1 % (15 min. ) And 70 % ethanol (1 min. ) Remained viable in culture conditions. The optimal concentration of auxin in combination with cytokines in the multiplication phase. The conditions for rooting induction in culture in vitro.
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