Comparison of Histological Changes of Recipient Wound Bed by Platelet Rich Plasma and Adipose Derived Stem Cell in Dogs

The aim of wound healing is to promote rapid wound closure and prevent excess scar formation. Open wound management, until the wound is considered suitable for reconstruction or until it has healed by second intention, has been the treatment of choice for centuries. Frequent and painful dressing changes over prolonged periods intensifies overall case management, and may impact treatment costs. Wound healing stimulated and optimum microenvironment for successful reconstruction that can be created by employing modern methods such as Platelet Rich Plasma and Stem Cell therapy. When skin was damaged, not only epithelial cells were destroyed, but also a large quantity of collagen was also lost. Currently, there is a growing interest in the use of autologous platelet concentrates (PC), also known as platelet rich plasma (PRP) for stimulating the healing process and promoting regeneration through growth factors instead of reparation (Mazzucco et al. , 2008). Stem cells and progenitor cells offer a potential answer to this dilemma (Kim et al. , 2012). Adipose derived stem cells (ADSCs) are an attractive and abundant stem cell source with therapeutic applicability in diverse fields for the repair and regeneration of acute and chronically damaged tissues. Several wound characteristics were considered that determined the level of histopatological changes e. g. depth and length of healed wound, epithelial stratification, incorporation of the dermal substitute, degree of neutrophil, macrophage, fibroblast, foreign body giant (FBG) cell infiltration and extent of elastin formation. Wounded tissue stained with Massons trichrome showed a clear view of collagen fibres deposition and reorganisation compared to Hematoxylin and Eosin staining. The present study was undertaken to compare and evaluate the wound healing between Platelet Rich Plasma and Adipose Derived Stem Cells.