Isolation and Antimicrobial Potential of Streptomycessp. LCC-06 from the Chennai Coast of Bay of Bengal, India
Journal: International Journal of Science and Research (IJSR) (Vol.5, No. 11)Publication Date: 2016-11-05
Authors : G. Laxshmi; Albin T. Fleming;
Page : 1102-1103
Keywords : Streptomyces sp; isolation; antimicrobial activity; drug discovery and marine soil;
Abstract
Streptomyces sp. is the largest genus of Actinobacteria known for producing antibacterial, antifungal, antiparasitic and immunosuppressant drugs. In the present study, Streptomyces sp. was isolated from the marine soil of Chennai, along the coasts of Bay of Bengal, India. After processing the soil sediment with calcium carbonate and heat, the suspension was inoculated in Starch casein agar medium by serial dilution method. The culture inoculate isolated from colony was identified as Streptomyces sp. and named as Streptomyces sp. LCC-06, where LCC represents Loyola College Chennai. Production medium-I was found to be the best media for antibiotic production. Secondary metabolite was extracted by mixing the culture broth free of biomass with equal volume (11, v/v) of ethyl acetate and methanol. The extract was dried in air and scrutinized for their antimicrobial activity. Gram positive and gram negative bacteria were cultured in Mueller-Hinton agar media and fungal pathogen was grown in potato-dextrose agar medium. They showed remarkable antagonistic potential for Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Klepsiella pneumonia, Escherichia coli, and Candida albicans. Marine soil in Chennai, along the coasts of Bay of Bengal hosts numerous potential antibiotic producing microorganisms like Streptomyces sp. LCC-06.
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