Isolation, Purification and Characterization of Arbutin from Cleidion nitidum (Muell. ? Arg.) Thw. ex Kurz. (Euphorbiaceae)

The methanolic leaf extract of Cleidion nitidum (Muell. Arg. ) Thw. ex Kurz. (Family Euphorbiaceae) was subjected to compound isolation and characterization. The extract was purified and isolated by column chromatography and thin layer chromatography (TLC). The isolated compound was then subjected to UV spectrum, FTIR for identification of functional groups and 1H-NMR and 13C-NMR for identification of protons and carbon atoms. ESI-MS was done to identify the molecular weight of the isolated compound. From the spectra obtained from FT-IR, 1H-NMR, 13C-NMR and ESI-MS, the isolated compound was found to be Arbutin (Hydroquinone -D-glucopyranoside) with the molecular weight of 272.25