THE INFLUENCE OF STRESS FACTORS ON THE ACTIVITY OF ENZYMES OF THE ANTIOXIDANT SYSTEM OF GUINEA PIGS LIVER WITH EXPERIMENTAL ALLERGIC ALVEOLITIS

Aim. Life in a modern metropolis has a negative efect on the human body. The causes of hepatic pathology are so numerous: bad ecology, fastfood, chronic stress, that there is a tendency to exaggerate them. And it happens because the liver provides neutralization of toxins, the effects of stress and unhealthy lifestyle, so widespread among residents of large cities. The goal of the research work was to study the level of prooxidant and antioxidant systems in the liver development during the experimental allergic alveolitis development. Material and Methods. Guinea pigs, males, weight 0,380-0,400 kg were employed in this experiment.The model of experimental allergic alveolitis was reproduced due to O.O.Orechova, Y.A Kyryllov method (1985): sensitization of animals by an injection of 0.2 ml of complete Freund's adjuvant into the hind leg. In two weeks after immunization the intravenously injection of 0.2 ml of a suspension of dead Bacillum Calmette Guerin was performed. On the 24th day of illness (early period) acute "immobilization stress" had been stimulated, after which the specimen (liver) was taken. Animals were immobilized on their backs with atraumatic fixation of the limbs on the operating table. The time of immobilization was 2 hours. The activity of lipid peroxidation was evaluated by the content of diene conjugates and malonic dialdehydi in the liver. The function of the antioxidant system was evaluated by the content of enzymes superoxide dismutase and catalase in the liver. Results and Discussion. In the experimental group we observed a significant increase in content of malonic dialdehydi, diene conjugates, and superoxide dismutase and catalase in comparison with the control group. In the experimental group of animals exposed to stress under experimental allergic alveolitis conditions we observed the following changes: a significant decrease in the content of diene conjugates and a significant increase in catalase activity . Comparing the group of animals with experimental allergic alveolitis and the experimental group of animals exposed to stress under experimental allergic alveolitis conditions, we observed a significant decrease in the content of malondialdehyde and diene conjugates ,and the activity of superoxide dismutase and catalase. Conclusions. 1. Results of biochemical studies indicate that experimental group of animals exposed to stress under experimental allergic alveolitis conditions present specific signs of oxidative stress.