NOVEL RPHPLC-PDA METHOD FOR SIMULTANEOUS ESTIMATION OF HEPARIN AND ITS CO-ADMINISTERED DRUG (FENOFIBRIC ACID) IN MIXTURE

The poly prescription and co-administration of more than two drugs to treat patients for the disease conditions are inexorable. Heparin is and anticoagulant often administered with fenofibric acid in cardiac treatments. A reverse phased high-performance liquid chromatography method based on photo diode array detection has been developed for their simultaneous determination from admixture. They were separated using potassium dihydrogen orthophosphate: acetonitrile (20:80, V/V), on C18 column, at a flow rate of 0.8 mL/min. The retention time of Heparin and fenofibric acid was 3.8 and 13.1 min, respectively. The validation parameters were studied as per ICH guidelines to ascertain the validity of the method for the purpose intended. The linearity was observed between 0.5-3 I.U./mL of heparin and 0.25-1.5 µg/mL of fenofibric acid. The method was applied to a synthetically prepared admixture, and found to be satisfactorily resolving two drugs without any interference, with good precision and reproducibility in the established condition.