SIMULTANEOUS ESTIMATION OF BERBERINE HYDROCHLORIDE AND INDOMETHACIN FOR IN VITRO DIFFUSION PROFILE BY UV SPECTROSCOPY

The present work aims to develop a precise, accurate and validation method to estimate berberine hydrochloride and indomethacin in gel formulations. In the present work, 50% phosphate buffer pH 7.4, 25% methanol and 25% ethanol blend was used as a solvent to increase the solubility of both the drugs. The analytical wavelength for berberine hydrochloride and indomethacin was 343 nm and 316nm, respectively. The method was validated for linearity, accuracy, precision, limit of detection, limit of quantification and robustness as per the ICH guidelines. The validated method was then applied in the diffusion study of the drugs from two different gel formulations. Beer's law was obeyed in the concentration range of 4-14 µg/ml and 10-60 µg/ml for berberine hydrochloride and indomethacin, respectively. The method was found to be accurate and precise with relative standard deviation within 2% as per the ICH guidelines.