GC-FID METHOD DEVELOPMENT AND METHOD VALIDATION OF EUGENOL IN CLOVE OIL EXTRACTED BY HYDRODISTILLATION

Eugenol is the essential component of many spice oils such as clove, cinnamon leaf, basil, nutmeg, etc. The determination of the authenticity of the essential oil is pivotal for estimating the purity of any essential oil. Hence, there is a high time to identify the marker compounds present in a particular essential oil. From ancient times, clove oil is known for many physiological properties. It is also widely used in dentistry and has potent antioxidant property. In this study, eugenol content in clove oil was evaluated by using GC FID. The ICH guideline was used to determine the suitability of the analytical method developed and validated.