PRELIMINARY PHYTOCHEMICAL SCREENING AND GC-MS ANALYSIS OF METHANOLIC LEAVES EXTRACT OF GLYCYRRHIZA GLABRA L. AND MALWA SYLVESTRIS L

India is one of the richest known in terms of biodiversity, especially the northern India Jammu and Kashmir one of the beautiful geographical regions of the world is hub of medicinal plants. Two medicinal plants namely Glycyrrhiza glabra L. and Malwa sylvestris L. were selected for the study. The aim of this research was to investigate presence of preliminary phytochemicals and to determine the total flavonoid and phenolic content. Isolation of phytoconstituents was analyzed through GC-MS. The solvent used was methanol and for the organic solvent extraction Soxhlet apparatus was used. The extract was then separated by GC-MS through Shimadzu (2010) model. Preliminary phytochemical screening of the methanolic leaves extracts of G. glabra L. and M. sylvestris L. was carried out using standard methods which showed alkaloids, flavonoids, glycosides, steroids, tannins, carbohydrates, proteins, phenols and anthraquinones respectively. Total phenolic content derived were 15.5mg/gm, 16.2mg/gm, 13.6mg/gm, 18.3gm/gm, 14.4mg/gm 13.6mg/gm, 24.3mg/gm, 19.5mg/gm, 21.3mg/gm and total flavonoid content derived were 10.2mg/gm, 23.4mg/gm, 18.5mg/gm, 19.3mg/gm, 24.3mg/gm, 19.8mg/gm, 22.4mg/gm, 25.6mg/gm, 16.2mg/gm respectively. GC-MS analysis showed forty two chemicals compounds from G. glabra L. and forty from M. sylvestris L. there were some major compounds noticed in both extracts which provided the evidence that these plants contain medicinally important bioactive compounds which can be used traditionally for the treatment of different kinds of fungal and bacterial diseases.