Novel Technology for Protein-Protein Interaction-based Targeted Drug Discovery
Journal: Journal of Analytical Science and Technology (JAST) (Vol.2, No. 3)Publication Date: 2011-12-15
Authors : Jung Me Hwang Kyung-Bok Lee Jong-Soon Choi Jaerang Rho Gun-Hwa Kim Seung Il Kim Soohyun Kim Zee-Won Lee;
Page : A61-A65
Keywords : Protein; Interaction; Translocation;
Abstract
We have developed a simple but highly efficient in-cell protein-protein interaction (PPI) discovery system based on the translocation properties of protein kinase C- and its C1a domain in live cells. This system allows the visual detection of trimeric and dimeric protein interactions including cytosolic, nuclear, and/or membrane proteins with their cognate ligands. In addition, this system can be used to identify pharmacological small compounds that inhibit specific PPIs. These properties make this PPI system an attractive tool for screening drug candidates and mapping the protein interactome.
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Last modified: 2012-11-07 23:19:17