Neurochemical Observations after the Transplantation of Human Amniotic Epithelial Cells in 2, 4, 5-trihydroxyphenylethylamine (6-OHDA) induced Parkinsonism Disease Model in Wistar albino rats

Background: In the present work the dopamine (DA) denervated rats exhibited vigorous contralateral rotational behaviour following apomorphine hydrochloride (APO Hcl) treatment. The 2, 4, 5-trihydroxyphenylethylamine (6-OHDA) lesioned animals showed significantly increased contralateral rotation. From the first week onwards the researcher observed changes in the lesion-induced rotational behaviour with the dopaminergic agnostic drug APO Hcl for fifty-sixty minutes from the time of administration. The lesioned animals showed more than 25.40 folds of rotations were taken for the Human Amniotic Epithelial (HAE) cells transplantation. Objective: To study the role of HAE cells in rotational behaviour and the level of dopamine and its metabolites indiscrete regions of the brain in 6-OHDA lesionedwistar albino rats. Material and Methods: Total of 48 Adult male Wistar albino rats weighing 180-220 gms were used for this study and were divided into four groups. They were maintained in a room at constant temperature and humidity (21ºC to 26ºC) and 12h light and dark cycle during the behavioural test and surgery. The DA and its metabolites were studied usinghigh-performance liquid chromatography with electrochemical detection (HPLC- ECD) method. Results: The HAE cells transplanted animals showed decreased contralateral rotation for first week 96%, second week 97%, third week 98% and fourth week 97% over the 6-OHDA lesioned animals. The level of DA and its metabolites in these animals were significantly increased in transplanted side than the contralateral side of 6-OHDA lesioned animals. The maximum significant increase was observed in striatum (DA 44.5 fold, 3,4-Dihydroxyphenylacetic acid (DOPAC) 44.9 fold and homovanillic acid (HVA) 1.9 folds) and minimum increase was in cerebral cortex (DA 30 %, DOPAC 2 fold and HVA 2 fold) among the brain regions studied after the HAE cells graft from the 6-OHDA lesion. Conclusion: Increase of DA and its metabolites in brain region after the HAE cells graft may therefore probably be dependent upon the diffusion of certain neurotrophic factors in the region of graft in the striatum and also due to intimacy of contact between the graft and the host striatum.