Evaluation Of Interleukin 6, Interleukin 8, TNF Alpha as Biomarkers For Pulpitis - In Vivo Stud

Introduction: Dental pulpitis occurs due to the inflammation of pulpal tissue. Inflammation of the pulp does not start when the bacteria reach the pulp rather the bacterial products may reach the pulp and initiate an inflammatory response. Aim: To evaluate the levels of interleukin 6, interleukin 8 and tumor necrosis factor alpha as biomarkers for reversible and irreversible pulpitis. Materials and Method: Saliva samples were collected from reversible and irreversible pulpitis and control patients. The samples were frozen until analysis. All samples were centrifuged for 15 minutes. The saliva supernatant was separated and stored at low temperatures. Cytokine profiling was determined using a sandwich immunoassay ELISA kits with 96- well plate. The specific cytokines- Interleukin 6, Interleukin 8 and Tumor necrosis factor Alpha were measured according to the manufacturer's protocol. Results: Data was tabulated in excel sheets and multiple anova tests was done. Samples collected from reversible and irreversible pulpitis showed higher cytokine levels than control levels. Samples collected from reversible pulpitis cases have shown to be associated with higher cytokines levels than the samples collected from irreversible pulpitis cases. Conclusion: Cytokine measurements may help in knowing the exact status of pulp and thereby predict the long-term prognosis of vital pulp therapies. Interleukin 6, interleukin 8, and tumor necrosis factor can be used as a biomarker for dental pulpitis.