Regenerative Capacity Of Resolvin D2 On Stem Cells Of Apical Papilla

Introduction: Regenerative endodontics is based on the triad of tissue engineering which has three components; stem cells, biomimetic scaffold and biomimetic growth factors which are introduced in to the canal space so as to induce pulp tissue regeneration and root maturation. Resolvins are lipid mediators that are released during the resolution phase of inflammation and regulate tissue repair. Thus the present study was conducted with the aim to evaluate whether resolvin D2 (RvD2) is capable of inducing hard tissue formation by its action on stem cells of apical papilla and compare it to concentrated growth factor (CGF). Materials and Methods: The root apical papilla tissues were carefully isolated from the root apex. Enzymatic separation was used for the cells of the primary apical papilla. The cells were subjected to the three groups namely RvD2, CGF and a combination of CGF and RvD2. Mineralized nodule formation was analyzed by alizarin red staining and dentin matrix protein 1 secretion was analyzed using ELISA test. Result: The combination of RvD2 and CGF had the highest values for mineralized nodule formation and DMP1 secretion followed by CGF. These were statistically more significant than the control group. However the results for RvD2 alone did not exceed that of CGF. Conclusion: Although RvD2 possesses regenerative potential and is capable of inducing stem cells of apical papilla for formation of mineralized hard tissue, its potential by itself does not surpass that of CGF. However the combination could be a promising new strategy in the management of immature necrotic permanent teeth.