Study To Evaluate Antimicrobial Efficacy Of Rubia Cordifolia Extract Against Cariogenic Organisms

Introduction: Streptococcus mutans, Lactobacillus acidophilus and Actinomyces viscosus have been identified as cariogenic organisms. Various mechanical and chemical aids have been designed to control the levels of these organisms. In view of instances of development of resistance to currently used chemicals or antibiotics and side effects, search for alternatives has led to herbal compounds being used as antimicrobials. Rubia cordifolia also known as Indian madder is a commonly used herb in Ayurveda for the treatment of various medical conditions. It is known for its antioxidant, anti inflammatory and antimicrobial activity. The aim of this study is to check the anti cariogenic activity of Rubia cordifolia against Streptococcus mutans, Lactobacillus acidophilus and Actinomyces viscosus. Methodology: Rubia cordifolia root powder was taken and methanol extract was prepared using Soxhlet apparatus. The bacterial strains of Streptococcus mutans, Lactobacillus acidophilus and Actinomyces viscosus were grown on Mueller Hinton Agar. The minimum inhibitory concentration and minimum bactericidal concentration was determined for the above mentioned organisms. Results: Rubia cordifolia extract was found to be effective against cariogenic organisms with a mean MIC of 12.5 mg/ml and MBC of 25 mg/ml. Conclusion: Rubia cordifolia extract can be used as an anticaries agent in mouth washes and dentifrices due to its effectiveness against cariogenic organisms.