Antimicrobial Activity Of Prunus Cerasus L Mouthwash - An In Vitro Microbial Study

Introduction: Mutans streptococci are the main cause of tooth decay. Application of natural materials as mouthwash has been effective in reducing the bacterial count. This study aimed to assess the antimicrobial effects of Sour cherry mouthwash on two oral bacteria S. mutans and S. sobrinus responsible for tooth decay. Materials and Methods: Strongly adherent strains of Streptococcus mutans and Streptococcus sobrinus were selected for this in vitro study. Antimicrobial effects of Sour cherry and Pomegranate mouthwash on microbial count in the biofilm and adhesion potential of bacteria were evaluated by microtiter plate method. Also, the well-plate technique was used to assess the effect of Sour cherry and Pomegranate mouthwash in comparison with CHX on bacterial growth and proliferation. The obtained results were analyzed by one-way ANOVA and Tukey's post hoc test using SPSS version 20.0 with Level of significance was set at P=0.05 with 95% confidence interval. Results: The chlorhexidine (CHX), Sour cherry and Pomegranate mouthwashes decreased plaque formation by S. mutans by 93%, 80% and 68%, respectively. These values for the S. sobrinus were 92%, 57% and 48%, respectively ( P<0.001). CHX was more effective than the other two materials (P<0.001). However, none of these materials eliminated the biofilm. Sour cherry and Pomegranate mouthwash inhibited the growth of the afore-mentioned bacteria. Conclusion: Within the limitations of this study, it showed that Sour cherry and Pomegranate mouthwash have the potential to prevent or control the proliferation of S. mutans and S. sobrinus