Green Synthesis of Copper oxide Nanoparticles Using Aqueous Extract of Ocimum sanctum and Analysis of Antimicrobial, Anti-inflammatory and Cytotoxic activity of Ocimum sanctum Copper Oxide Nanoparticles - An in vitro study

Introduction: Nanotechnology is one of the major research which has been going towards greater advances for research purposes. Nowadays nanoparticles have been used in medical research as the size of nanoparticle 100nm enters in the blood vessels, tissues in a shorter period of time. This might give a faster healing process .Ocimum sanctum which is a plant extract has medicinal values and it has been used in Asian countries which has an antioxidant agent. Materials and Methods: Ocimum sanctum powder extract is prepared and incorporated with copper oxide nanoparticles In which anti-inflammatory activity is done with diclofenac sodium with sample and standard preparation, antimicrobial activity was done in pathogenic strains of streptococcus mutans, streptococcus aureus, and lactobacillus strains and cytotoxic activity is tested with biologic organism of nauplii. Results: The anti-inflammatory activity shows the that Cuo nanoparticles shows little significance differences with standard preparation, antimicrobial activity shows the greater zone of inhibition in lactobacillus strains compared with streptococcus mutans and streptococcus aureus and cytotoxic activity shows the mortality rate of Cuo nanoparticles with ocimum sanctum in which 10 µl. Conclusion: The above results shows that ocimum sanctum with Cuo nanoparticles has shown that it has anti-inflammatory activity against the bacteria, antimicrobial activity has been seen in pathogenic strains to be known about and cytotoxic activity shows the mortality rate. The nanoparticles studies are being studied in the field of medicine due to action of particle size.