Cytotoxicity And Anti Microbial Analysis Of Graphene Oxide Decorated Silver Nanoparticles

Silver is known to have antimicrobial properties since decades.Graphene has various biomedical applications.Utilising plant extract for synthesis of nanoparticles appear promising and ecofriendly. Synthesis of graphene coated silver nanoparticles was done by utilising plant extract of Ocimum sanctum Linn and Andrographis paniculata as reducing agents.The characterisation was done by UV-vis spectrophotometer Analysis, Transmission electron microscopy, FTIR Analysis andX-ray Diffraction assay. Antimicrobial activity was assessed by measuring zone of inhibition(mm). Assessment of cytotoxicity was done by Brine shrimp lethality assay and MTT assay.The synthesised silver nanoparticles with an average size of 11-20 nm were uniformly dispersed on graphene sheets.The nanoparticles exhibited minimal cytotoxicity and good antimicrobial properties.Combination of Ocimum sanctum Linn and Andrographis paniculata were effective to be used as reduced agents for the synthesis of graphene oxide decorated silver nanoparticles.The synthesised nanoparticles appear as promising agents to be used as antimicrobial agents with minimal cytotoxic effects.