Novel Irrigant in Regenerative Endodontics - An In-Vitro Study

Introduction: Regenerative endodontic procedures have gained a limelight in recent times owing to the rates of success that has been reported by various authors. The potential regeneration of the tissues of the pulp-dentin complex yields us the hope to bring back the vitality of a non vital tooth. The management of a young permanent tooth with necrotic pulp has remained a challenge. In the present study, the potential of Farnesol, a sesquiterpene alcohol by origin with a good antimicrobial activity is tested for cytotoxicity, cell migration and cell proliferation assays against SCAP cells. Aim: Aim of the study was to evaluate if Farnesol could be used as an irrigant for necrotic immature permanent teeth. Materials and Methods: 95% Farnesol (Sigma Aldrich, US ) was bought. SCAP cells from Axol Pvt. ltd ( Cambridge, UK) were derived and a final concentration of 25µM/ml, 50µM/ml and 100µM/ml of the solution was tested on SCAP cells for the analysis of cytotoxicity, cell migration and cell proliferation. For cytotoxicity evaluation Sulforhodamine Assay, for evaluation of cell migration Transwell Migration Assay and cell proliferation In-Vitro Bromodeoxyuridine Assay were performed. Results: Analysis of the results showed statistically significant increase in cytotoxicity with the increase in the final concentration of Farnesol. Also, reduction in the cell migration and proliferation was seen to significantly decrease with the increasing concentration of the novel irrigant. Conclusion: Farnesol at a concentration lower than 25µM/ml, can be tried as a potential irrigant for the management of the necrotic immature permanent teeth by revascularisation technique. However, clinical studies need to be done to extrapolate this finding in reality