ASSOCIATION OF SERUM TNF- A WITH INSULIN RESISTANCE IN PRE-DIABETES AND TYPE 2 DIABETES MELLITUS

Background- First adipocyte derived biomarker is tumor necrosis factor alpha which connect the link between inflammation and diabetes. It expresses m-RNA which is contributing to the development of insulin resistance. However, the relationship between tumor necrosis factor alpha, pre-diabetes and Type2diabetes mellitus is still contradictory and restricted. Material & Methods: This study was hospital based analytical cross-sectional study. Total 900 subjects were distributed into three groups (300 pre-diabetic subjects, 300 type 2 diabetic subjects and 300 healthy subjects) as per ADA criteria. The biochemical parameters as Fasting Blood Glucose, 2-hr glucose (after 75 gm oral glucose intake), HbA1c and fasting insulin were analyzed. HOMA-IR was used to calculate insulin resistance mathematically. Anthropometric measurements were done. Tumor necrosis factor alpha was done by ELISA method. Results: Tumor necrosis factor alpha concentration was significantly increased in patients with type 2 diabetes mellitus and pre-diabetes in comparison to the control group at p value < 0.001 and showed a positive correlation with HOMA -IR. In conclusion, our findings suggested that systemic inflammation has an important role in the pathogenesis of pre diabetic condition. There is a progressive raises value of tumor necrosis factor alpha in pre diabetic and diabetic patients. Therefore, inflammation reflects the severity of the disease and signifies the presence of ongoing disease process.