Evaluation of Antimicrobial Activity of β-tricalcium Phosphate/Calcium Sulfate Mixed-up with Gentamicin: In-Vitro Study

Objectives: An in-vitro study was designed to investigate the antimicrobial activity of β-tricalcium phosphate/calcium sulfate in comparison to Xenograft, blended with either gentamicin or saline. Methods and materials: Employing Kerby-Bauer disk diffusion test,twelve Petri dishes (90 mm) with two models of culture mediums were used to cultivate three bacterial strains: Muller-Hinton Agar (for P. Aeruginosa/S. Aureus)andSheep Blood Agar (for Enterococcus faecalis).Then, studied materials were divided into four groups (Bovine, Bovine with gentamicin, EthOss and EthOss with gentamicin), mixed-upand transmittedinto their holes in each dish. After 24h of incubation, inhibition zones were noted and measured bya digital caliper.In the end statistical analysis were completed with One Way ANOVA followed by Tuckey HSD test on SPSS17. Results: One Way ANOVA and Tuckey HSD remarked astatically significant difference between all pairs for Enterococcus faecalis (p<0.05), except of Bovine/ EthOss pair. When it comes up to Staphylococcus aureus, a significant difference was observed between all pairs (p<0.05), except Bovine/ EthOss and EthOss Gentamicin/ Bovine Gentamicin pairs, both were effective In bacterial elimination. Finally, only Bovine Gentamicin was functional with Pseudomonas aeruginosa (p<0.05). Conclusion: β-tricalcium phosphate/calcium sulfate and Xenograft antibacterial abilities were improved when mixed with Gentamicin. Xenograft was preferable as an antibiotic carrier when it comes to Pseudomonas aeruginosa.