Hydroxyproline Levels in Gingiva and GCF in Patients with and without Chronic Periodontitis

Aim: The aim of this study was to compare hydroxyproline levels in both in the gingival tissue samples and gingival crevicular fluid from periodontally healthy subjects and chronic periodontitis subjects. Patients and Methods: A total of 40 individuals, 20 from each group, were studied. After recording clinical parameters, GCF was obtained without any contamination. The gingival samples were harvested by full thickness flap operation from periodontitis patients and immediately after the extraction of the teeth for orthodontic reasons from controls. ELISA analysis was performed to determine the hydroxyproline levels. Statistical Analysis: Mean values were compared by one way ANOVA and Mann-Whitney U test student t-test was used to compare continuous variables between two groups and Spearman correlation test was used to correlate hydroxyproline with clinical parameters for both the groups. A statistically significant p-value of 0.05 was used. Results: Clinical parameters in both the groups were positively correlated with hydroxyproline levels and higher concentrations of hydroxyproline were found in both GCF (844.5 ± 458.1 and 149.7 ± 62.2) and gingival tissue samples (1412.2 ± 627.7 and 217.4 ± 115.7) of group II subjects as compared with the group I subjects Conclusion: Hydroxyproline can serve as a biomarker in periodontal disease.