Association of Tumor Necrosis Factor-α Gene Promoter Polymorphisms with Periodontitisin Type II Diabetic Syrian Population

Objectives: Tumor necrosis factor-α (TNF-α) is a proinflammatory cytokine that can regulate periodontal tissue health and insulin-sensitive glucose uptake. TNF-α gene polymorphisms -857C/T and -1031T/C could influence periodontitisand type II diabetes mellitus (DM). The goal of this studyis to evaluate the association between TNF-α gene polymorphisms (-857C/T and -1031T/C) and both diseases in the Syrian population. Design: 180 subjects were recruited and allocated into four groups: H= healthy control, DM= diabetes mellitus, ChP= chronic periodontitis and ChPDM= chronic periodontitiswith DM.TNF-α SNPswereanalyzed by restriction fragment length polymorphism (RFLP-PCR) technique. Results: 857C/T gene polymorphism showed a lack of association with susceptibility to ChP, and T allele frequency was significantly higher in DM (adjusted OR 3.116, 95% CI: 1.184- 8.2, p = 0.019), Whereas, -1031CC genotype hadmore frequency in ChPDM group,and C allele has the higher probability in both diseases (OR= 3.013,CI: 1.275- 7.117, P = 0.011). Conclusion: Thisstudy data suggested that the TNF-α polymorphism -1031T/C couldbe a potentialrisk factors of periodontitis, but there were no association between -857C/T and periodontitis in diabetic patients in Syrian population.