Designation of bioindicator system for controlling the medical sterilization

Sterilization is one of the most effective methods to control hospital infections, and is applied according to the standards set by the international standardization organization. The sterilization method is selected according to the physical properties of the medical material. In this study, biological indicators were produced by evaluating the material and time parameters of the process. Geobacillus steorothermophilus is in spore form and was chosen for use in this study because it is widely used in making biological indicators. Escherichia coli was another bacterium selected for its vegetative form and its response time to low-temperature sterilization methods. Paired control was performed with two selected bacteria. Glucose and starch were used as nutrients for the growth of G. stearothermophilus and E. coli bacteria. It was tested which of the bacteria and nutrients gave faster results from the system prepared with the Arduino Uno and TCS3200 color sensor. According to the results of the study, it was seen that both bacteria were suitable for use as autoclave bioindicators and were sufficient for studies in terms of public health. The results obtained from this study showed that quality control processes can be done more accurately using a device, which read faster and more accurately than the human eye and be optimized and recorded with analytical data with its electronic and optical equipment.