Immunomorphological features of women's skin with androgenetic alopecia in the treatment of plasma enriched with platelets in combination with topical minoxidil 2 % lotion
Journal: The Journal of V.N. Karazin Kharkiv National University, series "Medicine" (Vol.43, No. 43)Publication Date: 2021-12-01
Authors : Olena Salyenkova Svitlana Danyliuk Yuliya Ovcharenko;
Page : 83-92
Keywords : androgenetic alopecia; platelet-rich plasma; minoxidil; immunohistochemical examination of skin biopsies;
Abstract
Introduction. The skin biopsies of 30 patients with androgenetic alopecia before and after treatment with platelet-rich plasma in combination with 2 % minoxidil were examined by immunohistochemistry. Goal. The study looked at immunohistochemical changes in the scalp biopsies of patients with stage I-II androgenetic alopecia according to the Ludwig scale and to identify possible changes after injection of platelet-rich plasma in combination with topical 2 % minoxidil lotion. Materials and methods. Skin biopsies of 30 patients with androgenetic alopecia were examined using an immunohistochemical study before and after treatment, which lasted 3 months and included 3 sessions of PRP (once per month) and topical application of minoxidil 2 % lotion twice a day. Results. It was found that androgenetic alorecia is accompanied by inflammatory perifollicular infiltration by T-lymphocytes CD3 +, CD4 + and CD8 +, macrophages (CD68 +); imbalance of germ polypeptides VEGF, TGF-β1, EGFR; accumulation of oxidative stress enzymes eNOS and iNOS; accumulation of pathological fraction of collagen IV. Injections of platelet-rich plasma in combination with topical administration of 2 % minoxidil as a treatment for androgenetic alopecia lead to normalization of immunohistochemical parameters of the skin, which indicates the possibility of using this combination for long-term therapeutic effect. Conclusions. This study complemented the understanding of the pathogenesis of AGA and serves as a basis for improving treatment regimens for this pathology. However, additional studies are needed to further study the pathomorphology of androgenetic alopecia and to standardize the technique of using platelet-rich plasma in patients with this disease.
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