Synthesis, Characterization and Biological Activity Studies of Semicarbazone Ligand

The ligand 1-(3-ethoxy-2-hydroxybenzilidene-4-phenylsemicarbazide), (L1) was synthesized and characterized with the help of Infra-red, Ultra-violet, 1HNMR spectroscopy and X-ray crystallography. The spectral data also indicate that the ligand coordinates through the phenolic oxygen and the azomethine nitrogen atoms. Crystal data revealed that the semicarbazone act as bidentate ligand, making use of azomethine nitrogen atom and oxygen atom for co-ordination to the central metal atom. The Ligand L1 have been screened for their antibacterial activity against gram positive bacteria Staphylococcus aureus and gram negative bacteria Escherichia coli and Pseudomonas aeruginosa.The ligand L1 exhibited appreciable activity against gram positive bacteria Staphylococcus aureus and it is resistant to the fungal species such as Candida albicans, Aspergillus niger and Macrophonia phaseolina. The free ligand L1also shows higher IC50 values against MCF-7 cells indicating the less anticancer activity.