Concentration of R-spondin 2 in the Follicular Fluid is Correlated with Implantation Rate, Estrogen and Amphiregulin, in Iraqi Women Undergo ICSI

Objective: to find out if there is any correlation between the concentration of R - spondin2, Amphiregulin, bone morphogenic protein - 15 in serum or follicular fluid, and serum estrogen before hCG administration (E2 before ICSI) and to investigate if there is a role of R - spondin2 in implantation rate of women who undergo ICSI Methods: A prospective study was conducted between the first of August 2015 to the third of April 2016, E2 was measured before hCG administration by vidas instrument using estrogen biomerieux kits for 45 women age ranged 18 - 42 years who will undergo ICSI and match the inclusion criteria, follicular fluid and serum were collected from those patients in the day of oocyte retrieval and then the concentration of Rspondin - 2, Amphiregulin, and bone morphogenic protein - 15 in follicular fluid and serum were measured using ELISA kit. Results: the concentration of F. R - spondin 2, S. R - spondin 2and F. AREG correlated positively with the concentration of E2 before ICS. The concentration of F. R - spondin 2, and F. AREGand E2 before ICSI. correlated positively with implantation rate. there was no difference in the SEM of the concentration of F. R - spondin 2, S. R - spondin 2and F. AREG, S. AREG, F. BMP - 15, S. BMP - 15 between pregnant and non - pregnant women. Conclusion: data of the present study show that R - spondin2 directly affected by the concentration of E2 and has a positive role in implantation rate that may forbid the early miscarriages after embryo transfer.