Glucose-Induced Oxidative Stress in Young and Old Erythrocytes: Modulatory Role of Grape Seed Proanthocyanidin Extract

Hyperglycemia is associated with increased oxidative stress (OS) and diabetes?related pathologies. Erythrocytes have a major role in the origin of various complications. We evaluated the responses of young and old erythrocytes from rats to induced-hyperglycemia along with the possible protection from grape seed proanthocyanidin extract (GSPE) against hyperglycemia. Erythrocytes obtained from percoll gradient were exposed to 10mM and 20mM glucose. Significant increases in fragility, ROS, Cai2+and MDA along with decreases in P-SH, GSH and ATP were partially reversed by GSPE. In conclusion, a natural polyphenol could possibly protect young erythrocytes against OS-related shortened lifespan and perhaps prevent hemolytic anemia.