BIOCHEMICAL AND ANTIOXIDANT ANALYSIS OF MADHUCA INDICA J.F GMEL

SRJIS/BIMONTHLY / M.RAJ AND S.PADHI ( 2 6 57 - 2 6 6 6 ) JAN ? FEB, 201 5. VOL - II I /XVI www.srjis.com Page 2657 BIOCHEMICAL AND ANTIOXIDANT ANALYSIS OF MADHUCA INDICA J.F GMEL. M. Raj , Department of Botany, Ravenshaw University, Cuttack - 753003, Odisha, India. S. Padhi , Department of Botany, Ravenshaw University, Cuttack - 753003, Odisha, India. *corresponding Author In recent time focus on plant research has increased all over the world and a large no ofevidence has collected to show immense potential of plants and their uses in various aspects. Madhuca indica J.F.Gmel. is commonly known as Mahua is an e conomically important plant belongs to family Sapotaceae. The goal of research work is comparative estimation of primary compounds such as total carbohydrate, reducing sugar, total lipid, total amino acids, protein, moisture and carotenoids from both dry a nd fresh corolla and assay of catalase, Peroxidase, and SOD activity from leaves. Highest amount of carbohydrate (602mg/g), reducing sugar (298mg/g) and protein (53.86mg/g) was observed in dry corolla, as compared to carbohydrate (213mg/g), reducing sugar (99.28mg/g), and protein (15.54mg/g) content of fresh corolla. Moisture content was high in fresh corolla (728mg/g) in contrast to dry corolla (110.62mg/g).Comparative account of total amino acid (16.4mg/g),carotenoids (33.39μg/g) content of dry corolla sh owed higher level than fresh corolla total amino acid (5.6mg/g), carotenoids (2.868μg/g).Dry corolla content lower amount of total lipid (22.6mg/g) as compared to fresh corolla (113.6mg/g).The antioxidant activity of the enzymes Catalase, peroxidase and su peroxide dismutase was 270unit - 1g, 7×103unit - 1g and 1.02unit - 1 in leaves.