Comparative Effect of Aqueous Aloe Vera Extracts on Oxidative Stress in Alloxan Induced Diabetic Wistar Rats

Background The main pathophysiology of diabetes mellitus DM is oxidative stress, which is an imbalance between production of free radicals and antioxidant system in the body. Persistent hyperglycemia causes increased production of free radicals especially reactive oxygen species ROS , in tissues from glucose auto oxidation and protein glycosylation. Aim To investigate the comparative effect of Aloe vera gel, rind and a combination of gel and rind on the oxidative stress in alloxan induced diabetic rats. Materials and Methods 25 male wistar rats weighing between 220-260g were used for this study. They were divided into five groups A E of 5 rats each. Group A served as positive control group and received distilled water only. Group B was the negative diabetic group with no extract administration. Groups C, D and E were diabetic groups that received 300mg kg of Aloe vera rind, 300mg kg of Aloe vera gel and combination of 150mg kg of Aloe vera rind and 150 mg kg of Aloe vera gel respectively. Extract administration was done orally, once daily for 14 days. At the end of administration, oxidative stress biomarkers were assessed. Results There was significant decrease in SOD level in group B 9.87±0.20 when compared with the control 15.88±0.39 . Conversely, groups C 17.50±0.35 and D 17.94±0.22 showed significant increase when compared with the control. However, group E showed no significant change when compared with the control 15.88±0.39 . On the other hand, there was significant increase in SOD in control group A 15.88±0.39 and all the test groups when compared with group B 9.87±0.20 . Similarly, a significant increase in MDA was observed in group B 5.39±0.20 when compared with the control 4.15±0.21 . Groups C 3.62±0.20 and D 3.23±0.12 showed significant decrease when compared with control 4.15±0.21 . Furthermore, group E 4.14±0.26 showed no significant difference when compared with the control 4.15± 0.2 . On the other hand, groups A and all the test groups showed significant decrease in MDA when compared with group B 5.39±0.20 . Conclusion Aloe vera gel and Aloe vera rind extracts singly appeared more potent in reducing oxidative stress in diabetic rats when compared to combined extracts of Aloe vera rind gel at lower doses. M. C. Igbokwe | V. U. Igbokwe | N. N. Njoku-Oji | C.O.J Okonkwo "Comparative Effect of Aqueous Aloe Vera Extracts on Oxidative Stress in Alloxan-Induced Diabetic Wistar Rats" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46381.pdf Paper URL : https://www.ijtsrd.com/humanities-and-the-arts/philosophy/46381/comparative-effect-of-aqueous-aloe-vera-extracts-on-oxidative-stress-in-alloxaninduced-diabetic-wistar-rats/m-c-igbokwe