Detection of high molecular weight IgG fibronectin complexes in various types of Acute Myeloid Leukemia - A study in third world country

To detect presence of high molecular weight com¬plexes of IgG and fibronectin, plasma of patients with Acute Myeloid Leukemia was examined by polyethylene glycol (PEG) precipitation, analytical ultracentrifugation, and immunoaffinity chromatography. Ultracen¬trifugation identified abnormal circulating high molecular weight IgG in all patients. This was precipitated by PEG and was shown by exclusion chromatography to contain IgG in a high molecular weight form. Exam¬ination of plasma by immunoaffinity chromatography supported evidence for complex formation between IgG and fibronectin and further showed that abnormal high molecular weight IgG complexes are a prominent feature of Acute Myeloid Leukemia and implicate IgG fibronectin complex formation.