Metabolic Profiling of Borrelia in Ticks and in Whole Blood Samples by Headspace Gas Chromatography - A Case Report | Biomedgrid
Journal: American Journal of Biomedical Science & Research (Vol.6, No. 4)Publication Date: 2019-11-26
Authors : Bruno Kolb; E Marion Schneider; Lorina Riesterer; Leona Bier; Tabea Hein;
Page : 340-347
Keywords : Borrelia; Lyme disease; Headspace gas chromatography; Antibiotics; Antibiotic resistance; garlic;
Abstract
The technique of static headspace gas chromatography (HS-GC) was applied to monitor the emission of hydrogen and carbon dioxide from bacterial cultures to detect bacterial contamination in ticks and the transfer to humans, causing Lyme borreliosis. While the standard serological methods to recognize Lyme-disease rely upon the indirect determination of antibodies, the present method enables the direct proof of bacteria both in ticks and in the blood of infected patients, due to the specific hydrogen emission. Moreover, the progress of an applied therapy can be monitored while antibodies, once created, remain in the blood regardless of whether the applied therapy was successfully finished or not. This method was also used to compare the efficacy of chemical and natural antibiotics. The most efficient natural antibiotics found were oil of cloves and garlic extract preparations. It was found that the duration of a successful therapy with chemical and natural antibiotics varied but can be perfectly monitored by the gas chromatographic technique.
Other Latest Articles
- Assessment of Patients’ Medical Laboratory Request Forms for Compliance in Jos University Teaching Hospital, Jos-Nigeria | Biomedgrid
- CHANGE IN LIFESTYLE AND DIETARY HABITS DURING COVID-19 PANDEMIC
- Influence of Primary Packaging on Attitudes towards Smoking in RN Macedonia | Biomedgrid
- Making a Case for use of Digital Interventions to Manage Adolescent Perinatal Depression in Nigeria | Biomedgrid
- Severe Allergic Reaction Caused by Pre-Operative Somatostatin Analog Treatment of Tsh- and Gh- Secreting Pituitary Adenoma: A Case Report | Biomedgrid
Last modified: 2022-03-10 15:04:35