ANTIBIOTIC EFFECTS OF SILVER NANOPARTICLES SYNTHESIZED FROM STYCHONS NUX VOMICA ETHYL ACETATE ROOT COLUMN FRACTION AGAINST CLINICAL RESISTANT STAPHYLOCOCCUS STRAINS

The present investigation was carried out to evaluate the antibacterial activity of leaf and root silver nanoparticles synthesized using different solvents. According to our studies the AgNPs of Strychons nux vomica synthesized from different solvent extract of root revealed to possess significant activity comparing to the antibacterial activity exhibited by AgNPs of leaf extract. Among the AgNPS tested, AgNPs synthesized using ethyl acetate extract showed highest inhibitory activity comparing to AgNPs synthesized using acetone and methanol extracts. The ethyl acetate extract based AgNPs showed highest zone of inhibition 29.2 mm noted against MRSA. Activity when compared with reference drugs used in the study, was found subtle low with the activity of Dicloxacillin (29.8mm) and high with Clindamycin (28.5mm). Leaf extracts exhibited antibacterial activity against clinically resistant S. aureus species. Among AgNPs tested, ethyl acetate extract, showed high growth inhibition activity against MMSA and MRSA with zone of inhibition 21.5 and 19.9 mm respectively. However, it is failed to inhibit the growth of VRSA. On the other hand, Ag NPs synthesized using acetone extract showed antibacterial activity against all three tested bacterial strains. The zone of inhibition 16.9, 17.8, 15.8mm was noted against MMSA, MRSA, VRSA respectively. Following to acetone extract Ag NPs, methanol extract Ag NPs are also exhibited antibacterial activity. The zone of inhibition 15.5, 12.1, 16.7 noted against MMSA, MRSA, VRSA respectively.