The Influence of the time and Temperature Binomium in the Process of Extraction of Bioactive Compounds from Guaraná (Paullinia cupana)

Guarana is a plant native to the state of Amazonas that found favorable soil and climate conditions for its development in southern Bahia. It is recognized worldwide for its medicinal and stimulant properties from the presence of alkaloids from the methylxanthine group, such as caffeine. The caffeine content in guarana can reach 6%, a content four times higher than arabica coffee, which makes it a useful raw material for the caffeine extraction process. Among the caffeine extraction processes, there is the method of extraction by chemical solvents, which, in addition to being expensive, are very harmful to the environment, so an alternative is the use of water as a solvent, which also has a good affinity in the process. extraction and does not harm the environment like chemical solvents. The objective of this project is to optimize the process of extracting caffeine from guarana seeds, using water as a solvent, through an experimental design. Chromatographic and microbiological analyzes will be carried out and the concentration of the extract will be performed with the highest yield by the spray drying method. Data evaluation will be performed using the Statistica software version 10, for analysis of variance with a 95% confidence interval. It is expected that, with this project, extracts rich in caffeine will be obtained and that it will be able to determine which variables influenced the caffeine extraction process, so that in this way they can be worked more deeply. Keywords: HPLC, Spray drying, Maltodextrin, Yield.