Stability Indicating RP-HPLC Method for Estimation of Cefquinome Sulphate in IV/IM Injection

The aim of present study was to develop and validate a simple, isocratic, accurate, stable, roust, and economical Reversed Phase High-Performance Liquid Chromatography (RP-HPLC) method for the determination of Cefquinome Sulphate in the IV/IM dosage form. The chromatographic separation was achieved on Inert sustain C18 column (250mm x 4.6mm x 5µ). The mobile phase selected was 25mM KH2PO4 buffer (pH 6.5) and Acetonitrile in the ratio of 80:20 % v/v at flow rate of 1.0ml/min with column temperature maintained at 40°C and 5µl injection volume was used. The detection was conceded out at 265nm.The developed RP-HPLC method yielded a suitable retention time of 4.2min for Cefquinome Sulphate. The method was linear under the range of 20 to 160 µg/mL with a linear regression coefficient of 0.9991. The %RSD for the method precision and system precision was found to be less than 2.0%. The %assay of formulation is 99.28%. The %Mean recoveries were discovered to be 100.29%. The LOD and LOQ was found to be 0.44 µg/mL and 1.34 µg/mL respectively. The specificity was calculated by using forced degradation studies in which the drug is subjected to stress conditions such as thermal, acidic, basic, oxidative and photolytic degradation. The developed and validated RP-HPLC method for Cefquinome Sulphate takes short time and can be used for routine Quality analysis of marketed Cefquinome Sulphate pharmaceutical dosage forms