Study the UV Spectra of Riboflavin (B2) Aqueous Solution under Different Stress Conditions

Riboflavin (B2) is a water-soluble vitamin that can be found in a variety of pharmaceutical and dietary products. In this the riboflavin standard aqueous solution was subjected to various conditions, including UV irradiation for 5, 10, 15, 20, 30, and 40 minutes, Temperature degrees at 30, 35, 40, 45, and 50?C, and 1 %, 5%, and 10% hydrochloric acid and sodium hydroxide. Its degradation is being studied using UV spectrophotometer. Riboflavin is UV sensitive after 20 minutes, thermostable up to 50?C, and unstable at varying acid and base concentrations. These methods can be used to investigate the stress degradation factors of riboflavin. An optimal pH with the most appropriate buffers would provide better vitamin stabilization in aqueous solutions.