ENHANCED EFFECTS OF CHOLECALCIFEROL PLUS PIOGLITAZONE ON LIPOLYSIS BY UP-REGULATION OF PPAR-ALPHA MRNA IN MATURE 3T3-L1 ADIPOCYTES

Introduction:Vitamin D deficiency has been associated with obesity, diabetes mellitus type 2, and hypertension diseases. The active vitamin D binds to nuclear vitamin D receptor (VDR) and regulating lipids and carbohydrates metabolism genes however, prophylaxis is ruled out because induce hypercalcemia and hyperphosphatemia. VDR and the peroxisome proliferator-activated receptor gamma (PPARγ) cross-talk regulates lipid and glucose metabolism genes. Methods: In this study was analyze the effect of cholecalciferol (CCF) as vitamin D plus pioglitazone (PIO) on mature 3T3-L1 adipocytes on proliferation, fat accumulation by colorimetric methods and the mRNA genes involved in lipid signaling by RT-PCR. Results: cellular proliferation in mature 3T3-L1 adipocytes treated with CCF plus PIO combination during 0.5, 6 and 24 hours, were not affected however, high CCF concentrations decreased cell proliferation. The combined treatment decreased fat accumulation, as well as increases glycerol release with CCF plus PIO-treated adipocytes. Key adipogenic genes were down-regulated (CEBP-α, SREBP-, PPARᵞ) whereas lipogenic genes (PPAR-α) were up-regulated at mRNA level. Conclusion: These findings imply that CCF plus PIO induce lipolysis through the expression of PPAR-alpha in adipocytes.