Formulation, Characterization and In vitro Evaluation of Dual-Drug Loaded Biomimetic Chitosan-Collagen Hybrid Nanocomposite Scaffolds

Background Diabetes Mellitus (DM) is one of the most concerning conditions, and its chronic complications are nearly synonymous with inflammation, oxidative stress, and infections. In the acute inflammatory phase of diabetic wound healing (DWH), reducing excessive reactive oxygen species (ROS) and inflammatory response of the wound is a necessary treatment. Methods The study utilizes a mix of emulsification and lyophilization approaches to investigate the effects of resveratrol microparticles (RES-GMS) loaded chitosan-collagen (CS-CLG) scaffold with doxycycline (DOX) on DWH. The RES-GMS was prepared using emulsification-linkage process. The prepared microspheres were evaluated for particle size, zeta potential, entrapment efficiency and SEM studies. Further, the RES-GMS was loaded in CS-CLG composite scaffold and crosslinked using EDC/NHS. The RES-DOX-CS-CLG scaffold was evaluated for morphology, porosity, water absorption test collagenase degradation, in vitro drug release, antibacterial activity, cell migration and in vitro cell biocompatibility. Results and Conclusion Cross-linked scaffolds had optimal porosity, reduced matrix degradation, and prolonged drug release when compared with non-cross-linked scaffolds, according to the results of composite scaffold characterization. Cell proliferation assay employing mouse fibroblasts was used to study the kinetics and bioactivity of growth factors produced from the scaffold. The RES-DOX-CS-CLG scaffold was biocompatible and promoted cell development compared with the control and CS-CLG scaffolds in in vitro experiments. DOX-loaded CS-CLG scaffold with RES-GMS delivered a prolonged release of RES, according to in vitro tests.