LC-MS/MS Method Applied to the Detection and Quantification of Ursodeoxycholic Acid Related Substances in Raw Material and Pharmaceutical Formulation
Journal: Journal of Pharmacy and Pharmacology (Vol.6, No. 5)Publication Date: 2018-05-05
Authors : Author s Boscolo Oriana; Flor Sabrina; Dobrecky Cecilia; Martinefski Manuela; Tripodi Valeria; Lucangioli Silvia;
Page : 448-455
Keywords : ;
Abstract
Objective: To develop a highly sensitive LC-MS/MS (liquid chromatography-mass spectrometry/mass spectrometry) method applied to the detection and quantitation of UDCA (ursodeoxycholic acid) related substances such as CA (cholic acid), DCA (deoxycholic acid), CDCA (chenodeoxycholic acid) and LCA (lithocholic acid) in raw material and pharmaceutical formulation. Methods: The method was validated for specificity, linearity, accuracy, precision, robustness. A triple quadrupole mass detector was employed, equipped with an ESI (electrospray ionization) source operated in the negative ion mode. The chromatographic system consisted of a Symmetry C18 column (150 mm × 4.6 mm, id; particle size 5 μm) and methanol-acetonitrile-ammonium acetate (pH 7.6; 10 mM) (40:40:20, v/v/v) as the mobile phase. The chromatographic conditions were 25 uL injection volume, flow rate of 0.4 mL/min and column temperature set at 35 °C. Key findings: The method requires a minimum sample amount and presents very low LOD (limits of detection) for CA (0.29 ng/mL), DCA (0.59 ng/mL), CDCA (0.13 ng/mL) and LCA (0.44 ng/mL) in comparison to LC methods coupled to different detectors like UV (ultraviolet), fluorescence and refractive index. Conclusions: The developed and validated LC-MS/MS method for the determination of UDCA and related substances in raw material and in a suspension was advantageous since it required a minimum sample amount. In turn, it could be used as a stability indicating method.
Other Latest Articles
- Stabilization of Alliinase from Garlic by Osmolytes and the Mannose-Specific Lectin ASAI
- Pharmaceutical Care in Homeopathic Treatment
- Excessively High Urinary Beta 2-Microglobulin Level: A Sign of Pathology or Laboratory Error ?
- Evaluation of the Effectiveness of Three Methods of Protecting Guinea Fowl from Mortalities Used in Traditional Breeding in Northern Togo
- Analysis of the Technical and Sanitary Constraints of the Traditional Breeding of Guinea Fowl in “Région des Savanes” of Northern Togo
Last modified: 2022-06-29 11:16:06