CARDIOPROTECTIVE EFFECT OF ENKEPHALINS UNDER IMMOBILIZATION STRESS

Objective: The aim of this study was to investigate the cardioprotective effect of dalargin, a synthetic leu-enkephalin. Methods: The induction of myocardial infarction in rats, which were kept on a diet with excess fat and calcium/sodium salts for two months, by the use of immobilization stress. The experimental results indicated that the applied model allowed to induce the development of myocardial infarction within one three days, which was confirmed by electrocardiography, enzyme-linked immunosorbent assay and histological examination. Results: Pre-treatment of rats with dalargin had no prevented myocardial infarction, however, it increased the resistance to immobilization stress and reduced infarction-induced myocardial lesions. Simultaneous administration of naloxone, an opiate receptor antagonist, together with dalargin eliminated its cardioprotective effect in experimental animals. Conclusion: The use of synthetic leu-enkephalin dalargin significantly reduced the risk of myocardial infarction caused by excessive neuromuscular stress. The dalargin effect on the myocardium was mediated by opiate receptors.