CHARACTERISTICS OF INDICATORS OF MICROBIOLOGICAL EFFICACY OF ALCOHOL-CONTAINING AGENTS

The purpose of the research was to study the indicators of microbiological efficacy of compositions of ethyl alcohol 72% with brilliant green 0.01-0.001%, crystalline iodine 0.5-0.1%, chlorhexidine bigluconate 0.5-0.01%. Material and methods. The studies were performed on standard test cultures of Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Proteus mirabilis ATCC 14153, Candida albicans ATCC 10231, as well as on clinical strains of Klebsiella pneumoniae 620, Acinetobacter baumannii 445, isolated from patients of the Vitebsk Regional Clinical Infectious Diseases Hospital, and clinical strains of Klebsiella pneumoniae 1051, Acinetobacter baumannii 886, Staphylococcus aureus 1230, Pseudomonas aeruginosa 1074, isolated from patients of the Vitebsk Regional Clinical Hospital. Results. In the studied alcohol compositions the totalamountofaerobesislessthan 102 CFUper 1 ml, the totalnumber of fungi is less than 10 CFU per 1 ml. Standard and clinical strains of microorganisms are sensitive to compositions of ethyl alcohol 72% with all the studied concentrations of brilliant green, crystalline iodine, chlorhexidine bigluconate, as well as ethyl alcohol 70% and 72% at an exposure of 1 minute in a qualitative suspension test tube method without protein load, in a micromethod on sterile 96-dimple polystyrene plates and in the disk diffusion method. The reduction factor in the quantitative suspension method for all the studied compositions in relation to standard and clinical strains is above 5.0 lg. Conclusions. The results of the study enable to conclude that the developed alcohol compositions have high antimicrobial activity against standard and clinical strains and meet the standard microbiological indicators of the efficacy of disinfectants and antiseptics, and are microbiologically pure and meet the regulatory requirements in terms of microbiological purity. Compositions of ethyl alcohol 72% with brilliant green 0.01%, with crystalline iodine 0.25%, with chlorhexidine bigluconate 0.1% are combined agents with a sufficient synergistic effect and can be recommended as prophylactic antimicrobial agents.