STATE OF PROOXIDANT AND ANTIOXIDANT BLOOD SYSTEMS IN WHITE RATS WITH EXPERIMENTAL DIABETES MELLITUS

Aim of the research:to study the state of prooxidant-antioxidant blood systems in white rats with experimental diabetes mellitus. Recent researcheshave shown that one of the important links in the pathogenesis of diabetes is a violation of the processes of free radical oxidation of lipids and weakening of antioxidant defenses. It is known that the primary products of lipid peroxidation are diene conjugates -the result of oxidation of polyunsaturated higher fatty acids at the stage of formation 431(21) січень-березень, 2022ISSN2521-1455 (Print)ISSN2523-4250 (Online) «Art of Medicine»and realization of free radicals that have a damaging effect on lipids, proteins and enzymes. Under the conditions of lipid peroxidation processes activation, the functional activity of intracellular defense systems is thegreat importance, which includes the antioxidant system -a complex of enzymatic and non-enzymatic antioxidants. The key enzyme of the antioxidant system is catalase, which belongs to the first line of defense against reactive oxygen species. Therefore, the study of the dynamics of prooxidant-antioxidant status in experimental diabetes will be able to provide pathogenetically justified corrective measures.Materials and methods.The experiments were performed on 88 white male Wistar rats weighing 170-210 g, that were kept on a standard diet with free access to wa-ter. Animals were divided into three groups: 1 -intact (n = 10); 2 -control (n = 40); 3 -experimental (n = 38) with a model of diabetes mellitus, which was reproduced by in-traperitoneal injection of streptozotocin by “Sigma" com-pany (USA), diluted in 0.1 M citrate buffer with a pH of 4.5, at a rate of 60 mg / kg body weight. The control group of animals received an intraperitoneal injection with an equivalent dose of 0.1 M citrate buffer solution with a pH of 4.5.Serum levels of diene conjugates and catalaseac-tivity were measured on 14th, 28th, 42nd and 70th day af-ter streptozotocin injection.Results.The conducted biochemical analysis showed that 14 days after diabetes modeling there was an increase in serum of diene conjugates levels by 31.8% (p˂0.001) and catalase concentration by 46.6% (p˂0.001) relative to the control group of animals. After 28days of the experiment, a further increase in the concentration of diene conjugates by 104.4% (p˂0.001) and catalase activ-ity by 74.8% (p˂0.001) was determined,compared to the control group of animals. With increasing study time (42 days), the level of primary products of lipid peroxidation in serum increased by 112.5% (p˂0,001). Theactivityofserumcatalasewasincreasedby29.3% (p˂0.01). After 70 days, the level of diene conjugates increased and exceeded the control value by 125.4% (p˂0.001). At the same time, there was a decrease in catalase activity by 28.1% (p˂0.01) in serum compared to the control group of animals.Conclusions.Experimental diabetes mellitus is accompanied by an intensification of lipoperoxidation pro-cesses throughout the study period, which is manifested by a significant increase in serum levels of diene conjugates. On the background of diabetes mellitus development there is depletion of the enzymatic link of the antioxidant system in the serum, as evidenced by a reduction in catalase activ-ity, which is especially expressed on the 70th day of the experiment.