Analytical Method Development by High Performance Liquid Chromatography

HPLC is the dominant separation technique in modern pharmaceutical and biomedical analysis because it results in highly efficient separations and in most cases provides high detection sensitivity. Most of the drugs in multi component dosage forms can be analyzed by HPLC method because of the several advantages like rapidity, specificity, accuracy, precision and ease of automation in this method. HPLC methods development and validation plays an important role in new discovery, development, manufacture of pharmaceutical drugs and various other studies related to humans and animals. This review gives information regarding various stages involved in development and validation of HPLC method. Validation of HPLC method as per ICH Guidelines covers all the performance characteristics of validation, like Accuracy, Precision, Specificity, Linearity, Range and Limit of detection, Limit of quantification, Robustness and system suitability testing. Tanmayi Kalamkar | Tejaswini Kande | Naziya Sayyad | Dipti Patil "Analytical Method Development by High Performance Liquid Chromatography" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-6 | Issue-4 , June 2022, URL: https://www.ijtsrd.com/papers/ijtsrd50177.pdf Paper URL: https://www.ijtsrd.com/pharmacy/analytical-chemistry/50177/analytical-method-development-by-high-performance-liquid-chromatography/tanmayi-kalamkar