Development and Validation of HPLC Method for Estimation of Gliclazide in Gliclazide Tablets Prepared using Natural Disintegrant

A simple, selective, linear, precise and accurate HPLC method was developed and validated for the estimation of Gliclazide in Gliclazide tablets prepared using natural super disintegrant by direct compression method. Isocratic elution at a flow rate of 1.0 mL min was employed on HiQSil C18 250 mm × 4.6 mm, 5 µm column at ambient temperature using Methanol Phosphate Buffer 60 40 v v as mobile phase. The UV detection wavelength was carried out at 210nm. The linearity of the developed method was studied over the concentration ranges between 10 30µg ml. The retention time for Gliclazide was 3.2 min. The developed method was validated as per the ICH guidelines with respect to system suitability, specificity, precision, accuracy and robustness. The Minimum concentration level at which the analyte can be reliable detected LOD and quantified LOQ were found to be 0.54 and 1.15 µg ml respectively. The proposed method can be successfully applied for the estimation of Gliclazide in pharmaceutical dosage forms. Mr. Kiran Madde | Dr. Ravindra Patil | Dr. Amit Kasbe "Development and Validation of HPLC Method for Estimation of Gliclazide in Gliclazide Tablets Prepared using Natural Disintegrant" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-6 | Issue-4 , June 2022, URL: https://www.ijtsrd.com/papers/ijtsrd50403.pdf Paper URL: https://www.ijtsrd.com/humanities-and-the-arts/education/50403/development-and-validation-of-hplc-method-for-estimation-of-gliclazide-in-gliclazide-tablets-prepared-using-natural-disintegrant/mr-kiran-madde