Optimization of Metalloprotease Enzyme Extracted from Marine B.thuringiensis Strain 1257 Metabolism
Journal: International Journal of Science and Research (IJSR) (Vol.11, No. 6)Publication Date: 2022-06-05
Authors : Maha AL Rahhal; Joumaa Merza;
Page : 1374-1380
Keywords : Marine Microbial Protease; Metalloprotease; B. Thuringiensis; EDTA;
Abstract
Marine water contains large numbers of different species of microorganisms particularly, bacteria, which is considered to be a biological factory for producing protease enzymes that offer great biotechnological, industrial and pharmaceutical applications. At this work we isolated a hyper protease producer bacterium B.thuringiensis strain 1257 from the Mediterranean Sea water and a purified protease from strain 1257. The protease was identified as metalloprotease. Metalloprotease was partially purified by ammonium sulfate followed by application on Sephadex G-75 column. Gel filtration step resulted in more than 40 times fold purification of the purified enzyme. The enzyme activity was inhibited by EDTA (almost 80%) at 15 mM concentration. Optimum temperature for enzyme activity was 60 ?C. It also exhibited a broad pH activity range (6-9) with an optimum pH of 8.
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Last modified: 2022-09-07 15:17:07