Isolation and Characterization of Un-Veiling Potential of Bio-Surfactant Isolated from Oil Contaminated Region near Petrol Pump

Biosurfactant are surface active surfactant with wide spectrum of applications in varied industrial sectors such as cosmetics, pharmaceuticals, biomedical, food industries, microbial enhanced oil recovery and agriculture. Thirty-three distinct microbial colonies were isolated form petrol pump, collected from Kanpur region, for screening most potent biosurfactant strains. Primary screening methods such as emulsification index (E24), drop-collapse assay, oil-spreading method, oil displacement method, CTAB (Cetyl Tri Ammonium Bromide) were conducted. Biosurfactant were further characterized by using Thin Layer Chromatography (TLC). The present study is concentrated on the utility of bio surfactants for its antibiotic potential against microbes capable of deteriorating crop production. Since the antibiotic character was driven from the environmentally safe metabolite of oil contamination causing microbes. The results showed that out of three, two isolates SL1 and SL3 showed potential of bio-surfactant production and their relative antagonistic property against Campylobacter jejuni, Listeria monocytogenes and Staphylococcus aureus.