Yeast Mediated Synthesis of Iron Oxide Nano Particles: Its Characterization and Evaluation of Antibacterial Activity

Production of nanoparticles by using microbes are a new area of interest. Saccharomyces Cerevisiae (baker's yeast) was allowed for the biosynthesis of iron nanoparticles in a culture media. Myco-synthesis of iron nanoparticles is considered to be more straight forward and easy for the stable production of NPs as compared to bacteria. Fungi have several advantages over bacteria showing (i) higher biomass and easy mode of culture, (ii) higher bioaccumulation of metabolites (iii) higher tolerance to, and uptake capability of metals. Present paper discussion “Biosynthesis, characterization and evaluation of iron nanoparticles by yeast cells”. It was carried out by culturing the yeast powder and the yeast obtained from the potato water on PDA (potato dextrose agar media) and incubated for 1-2 days. After incubation, and subcultured on PDA slants under sterile conditions. Inoculum was prepared by transferring the cells from PDA slants into 250 ml flask containing a mixture of ferrous sulphate (100mM) and ferric chloride (0.001). Four different culture media was prepared in four different flasks. The flasks is then kept in the rotary shaker at 30 °C at 120RPM for 2-3days. End of the synthesis the cells and debris were removed by centrifugation/filtration. The residue was collected and dried to get the dry powder of iron nanoparticles. Progressive results and maximum yield of iron nanoparticles obtained with Trial-II. Further the biosynthesized Iron nano particles were characterized by analytical techniques like UV-Visible Spectrophotemetry, FT-IR Spectroscopy and SEM studies. The UV-Visible absorption spectrum showed an absorption maxima at 249 nm which is a characterstic of iron nanoparticles. The FT-IR spectrum of iron nanoparticles showed absorption peaks ranging between 3439.19 cm-1 to 542.02 cm-1 range. SEM images showed 155.7 nm of spherical shape. Iron nanoparticles showed anti-microbial activity against both gram –ve , gram +ve bacteria, but Iron nanoparticles showed more activity against the Bacillus sps than E.coli.