Prevalence and Molecular Detection of Bacteria Isolated from Sexually Transmitted Disease in Al-Anbar Province

This study is the first in the Al-Anbar governorate with this design aim to the detection of Sexually transmitted diseases (STDs) by using RT-PCR. A total of (70) samples were obtained from women18 years of age and over, with or without symptoms of genital tract infection, randomly collected and carried out intermittently in Al-Anbar province between march 2020 and January 2021.  STIs bacteria were detected by Real-time PCR. Out of 70 samples with or without symptoms of genital tract infection for STDs, bacteria detection using real-time PCR technique. The results were 36 (51%) and 34 (48.5%), negative and positive, respectively. The results revealed four genera of sexually transmitted bacteria causative agents of sexually transmitted diseases. These included bacteria from Ureaplasma parvum that accounted for the highest percentage of infections with sexually transmitted diseases, with (20) 28% of 34 (48.5%) positive results. Although Mycoplasma genitalium, was the lowest at (2) 2.8%, detecting two other bacteria including Chlamydia trachomatis, was identified with a rate of (6) 8.5%, and Mycoplasma hominis bacteria was identified with the same percentage. The results supported the need for greater STI prevention knowledge. Seven STI pathogens were successfully identified in our research population using the real-time PCR approach. The Mycoplasma genitalium pathogen had the lowest detection rates compared to the typically high Ureaplasma parvum pathogen detection rates.